GJA1 Rabbit Polyclonal Antibody

CAT#: TA328600

Rabbit Polyclonal Anti-Connexin-43

Product Images

Western blot analysis of mouse brain membranes: 1. Anti-Connexin-43 antibody (1:400). 2. Anti-Connexin-43 antibody, preincubated with the control peptide antigen.

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  Western blot analysis of rat heart membranes: 1. Anti-Connexin-43 antibody (1:400). 2. Anti-Connexin-43 antibody, preincubated with the control peptide antigen.

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  Expression of Connexin-43 in rat cerebellum. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Connexin-43 antibody (1:300). Connexin-43 staining (red) appeared in Bergmann glial fibers (arrows) in the molecular layer (MOL) and in the granule layer (G). Cell nuclei were stained with DAPI (Blue).

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  Expression of Connexin-43 in rat intestinal epithelial IEC-6 cells. Immunocytochemical staining of fixed and permeabilized rat intestinal epithelial IEC-6 cells. Cells were stained with Anti-Connexin-43 antibody (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Cell nuclei were visualized using Hoechst 33342 (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gap junction protein alpha 1
• Database Link:
  NP_000156
  Entrez Gene 14609 MouseEntrez Gene 24392 Rat
  UniProt ID P17302
• Background: Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities.
• Synonyms: AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS; ODDD; PPKCA

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction