CHRM1 Rabbit Polyclonal Antibody

CAT#: TA328609

Rabbit Polyclonal Anti-M1 Muscarinic Receptor (443-458)

Product Images

Western blot analysis of rat brain lysate: 1. Anti-M1 Muscarinic Receptor (443-458) antibody, (1:200). 2. Anti-M1 Muscarinic Receptor (443-458) antibody, preincubated with the control antigen.

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  Expression of M1 receptor in mouse striatum. Immunohistochemical staining of mouse striatum using Anti-M1 Muscarinic Receptor (443-458) antibody, (1:100). M1 Muscarinic Receptor staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 Muscarinic Receptor. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cholinergic receptor muscarinic 1
• Database Link:
  NP_000729
  Entrez Gene 12669 MouseEntrez Gene 25229 RatEntrez Gene 1128 Human
  UniProt ID P11229
• Background: The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5. The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues. Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control. They also participate in learning and memory processing. The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors are also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves. The m1, m3 and m5 receptors, which are coupled to Gq/11 proteins, can protect cells from apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m1, m3 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.
• Synonyms: HM1; M1; M1R

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton