TRPC5 Rabbit Polyclonal Antibody

CAT#: TA328641

Rabbit Polyclonal Anti-TRPC5

Product Images

Western blot analysis of rat brain membranes: 1. Anti-TRPC5 antibody, (1:200). 2. Anti-TRPC5 antibody, preincubated with the control peptide antigen.

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  Expression of TRPC5 in rat cortex. Immunohistochemical staining of rat cortex using Anti-TRPC5 antibody. A. TRPC5 immunoreactivity (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel subfamily C member 5
• Database Link:
  NP_036603
  Entrez Gene 22067 MouseEntrez Gene 140933 RatEntrez Gene 7224 Human
  UniProt ID Q9UL62
• Background: The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels. The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) TRPC3, TRPC6, TRPC7 4) TRPC2. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC5 channels are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells, and can be activated either by calcium store depletion or by GPCR stimulation pathways.
• Synonyms: PPP1R159; TRP5

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane