P2Y12 (P2RY12) Rabbit Polyclonal Antibody

CAT#: TA328649

Rabbit Polyclonal Anti-P2Y12 Receptor (extracellular)

Product Images

Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate: 1-2. Anti-P2Y12 Receptor (extracellular) antibody, (1:200). 3-4. Anti-P2Y12 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of P2Y12 in rat RBL cells. Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells using. A. Extracellular staining of cells using Anti-P2Y12 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.

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  Indirect flow cytometry analysis of MEG-O1 living cells: black line: Unstained cells. Green line: Cells + Anti-P2Y12 Receptor (extracellular) antibody, (5-10 ug/1x106 cells).

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:200-1:2000; FC: 1:50-1:600
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2Y12 receptor. 3rd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: purinergic receptor P2Y12
• Database Link:
  NP_073625
  Entrez Gene 64803 RatEntrez Gene 64805 Human
• Background: The P2Y receptors belong to the G-protein coupled receptors superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA. Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14. The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.
• Synonyms: ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane