TRPM8 Rabbit Polyclonal Antibody

CAT#: TA328652

Rabbit Polyclonal Anti-TRPM8 (extracellular)

Product Images

Western blot analysis of prostate carcinoma cell lines; DU145 (lanes 1, 3), LNCaP (lanes 2, 4) and mouse-TRPM8 transfected HEK-293 (lanes 5, 6) cell lysates : 1, 2, 5. Anti-TRPM8 (extracellular) antibody, (1:200). 3, 4, 6. Anti-TRPM8 (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of TRPM8 in rat DRG. Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) antibody, (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.

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  Expression of TRPM8 in LNCaP prostate carcinoma cell line. Immunocytochemical staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody. A. Extracellular staining of intact LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:100), followed by goat anti-rabbit-AlexaFluor-550 (red), (x100). B. Intracellular staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:1000), followed by goat anti-rabbit-AlexaFluor-488 (green), (x100).

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  Immunocytochemical staining of intact living rat root dorsal root ganglion (DRG) cells. A. Intracellular staining of cells with Anti-TRPM8 (extracellular) antibody, (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Extracellular staining of live cells with Anti-TRPM8 (extracellular) antibody (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8. 3rd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel subfamily M member 8
• Database Link:
  NP_076985
  Entrez Gene 171382 MouseEntrez Gene 171384 RatEntrez Gene 79054 Human
  UniProt ID Q7Z2W7
• Background: The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1 The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7. The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures((~28°C) or menthol. TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel. In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive. In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.
• Synonyms: LTRPC6; TRPP8

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane