HRH4 Rabbit Polyclonal Antibody

CAT#: TA328667

Rabbit Polyclonal Anti-Human H4 Histamine Receptor (extracellular)

Product Images

Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: 1, 2. Anti-Human H4 Histamine Receptor (extracellular) antibody, (1:400). 3, 4. Anti-Human H4 Histamine Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  Indirect flow cytometry analysis of H4 Histamine Receptor expression in live intact Jurkat (acute T-cell leukemia) cell lines. black line, Unstained cells + goat-anti-rabbit-PE. green line, Cells+ Anti-Human H4 Histamine Receptor (extracellular) antibody, (1:20) + goat-anti-rabbit-PE.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human H4 Histamine Receptor. 1st extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histamine receptor H4
• Database Link:
  NP_067637
  Entrez Gene 59340 Human
  UniProt ID Q9H3N8
• Background: Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion. The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H4 Histamine Receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade. H4 Histamine Receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 Histamine Receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 Histamine Receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production. These studies indicate that H4 Histamine Receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.
• Synonyms: AXOR35; BG26; GPCR105; GPRv53; H4; H4R; HH4R

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction