Cacna1b Rabbit Polyclonal Antibody

CAT#: TA328722

Rabbit Polyclonal Anti-CaV2.2

Product Images

Western blot analysis of rat brain membranes: 1. Anti-CaV2.2 antibody, (1:200). 2. Anti-CaV2.2 antibody, preincubated with the control antigen.

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  Expression of Cav2.2 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum with Anti-Cav2.2 antibody, (1:100). A. Cav2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje cells with mouse anti-Calbindin 28K (green) demonstrates the restriction of Cav2.2 to cell bodies but not to dendrites in the molecular layer. C. Merged image of panels A and B.

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  Expression of Cav2.2 in rat DRG primary culture. Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Cells were stained using Anti-Cav2.2 antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat Cav2.2. Intracellular loop between domains II and III.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: calcium voltage-gated channel subunit alpha1 B
• Database Link:
  NP_671482
  Entrez Gene 774 HumanEntrez Gene 12287 MouseEntrez Gene 257648 Rat
• Background: Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.The voltage dependent Ca2+ channels are composed of several subunits; a1, Ã?, a2d and ?. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type.The CaV2.2 (formally named a1B) composes the a1 poreforming subunit for the N type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, ?-aminobutyric acid, acetylcholine, dopamine and noradrenaline.The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding GÃ?? to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.?-Conotoxin GVIA is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 a1 subunit (a1B) and its action is only partially reversible.
• Synonyms: BIII; CACH5; CACNL1A5; CACNN; Cav2.2