TRPV5 Rabbit Polyclonal Antibody

CAT#: TA328743

Rabbit Polyclonal Anti-TRPV5

Product Images

Western blot analysis of rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates: 1, 2. Anti-TRPV5 antibody, (1:200). 3, 4. Anti-TRPV5 antibody, preincubated with the control peptide antigen.

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  Expression of TRPV5 in rat kidney. Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 antibody, (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.

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  Expression of TRPV5 in PC3 cells. Immunocytochemical staining of paraformaldehyde-fixed PC-3 cells with Anti-TRPV5 antibody, (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Same as in (A) but pre-treated with TRPV5 peptide antigen (1:20). Hoechst 33342 is used as the counterstain (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel subfamily V member 5
• Database Link:
  NP_062815
  Entrez Gene 116469 RatEntrez Gene 194352 MouseEntrez Gene 56302 Human
  UniProt ID Q9NQA5
• Background: TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP). The TRPV subfamily consists of six members, TRPV1-6. TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dIHCydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV5 is preferentially expressed in kidney.
• Synonyms: CAT2; ECAC1; OTRPC3

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane