Cacnb1 Rabbit Polyclonal Antibody

CAT#: TA328764

Rabbit Polyclonal Anti-CaVBeta1

Product Images

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat cortex (lane 3 and 6): 1-3. Anti-CaVβ1 antibody, (1:800). 4-6. Anti-CaVβ1 antibody preincubated with the control peptide antigen.

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  Expression of CaVβ1 in rat hippocampus. Immunohistochemical staining of rat hippocampus using Anti-CaVβ1 antibody. A. CaVβ1 staining (red) appears in dendrites of the stratum radiatum (arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of A and B.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CaVÃ?1. Intracellular, adjacent to the C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: calcium voltage-gated channel auxiliary subunit beta 1
• Database Link:
  NP_059042
  Entrez Gene 12295 MouseEntrez Gene 50688 Rat
  UniProt ID P54283
• Background: Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, CaV channels have traditionally been classified into L, T, N, P, Q, and R types. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming a1 subunit, which triggers Ca2+ flow across the membrane, and the subunits a2d, ?, and Ã?.CaVÃ? subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The CaVÃ? subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the a1-subunit, which allows channels to traffic to the surface membrane4. Furthermore, CaVÃ? subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane. There are four distinct CaVÃ? subunits CaVÃ?1, CaVÃ?2, CaVÃ?3 and CaVÃ?4.Three splice variants exist for the Ã?1 subunit: Ã?1a, Ã?1b and Ã?1c. Ã?1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. Ã?1a appears to be important for the functional expression of the a1 subunit in skeletal muscle. Ã?1b was identified by cloning in rat brain, heart and hippocampus, and differs from Ã?1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. Ã?1c was cloned from human heart and hippocampus and has the same deletion as Ã?1b, but lacks the C-terminal extension.
• Synonyms: CAB1; CACNLB1; CCHLB1; MGC41896