Trpc1 Rabbit Polyclonal Antibody

CAT#: TA328769

Rabbit Polyclonal Anti-TRPC1 (extracellular)

Product Images

Western blot analysis of rat brain lysate: 1. Anti-TRPC1 (extracellular) antibody, (1:200). 2. Anti-TRPC1 (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of TRPC1 in PC12 cells. Immunocytochemical staining of intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC1 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining with DAPI as the counterstain. C. Merged images of A and B.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:200-1:2000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1. 2nd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel, subfamily C, member 1
• Database Link:
  NP_446010
  Entrez Gene 7220 HumanEntrez Gene 22063 MouseEntrez Gene 89821 Rat
  UniProt ID Q9QX01
• Background: The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels. The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities: 1.) TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3, TRPC6, TRPC7 4.) TRPC2. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC1 was the first mammalian TRP protein that was reported to form an ion channel. It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet. The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+, store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.
• Synonyms: HTRP-1; MGC133334; MGC133335; TRP-1; TRP1
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.