Trpv4 Rabbit Polyclonal Antibody

CAT#: TA328772

Rabbit Polyclonal Anti-TRPV4 (extracellular)

Product Images

Western blot analysis of rat brain lysate: 1. Anti-TRPV4 (extracellular) antibody, (1:200). 2. Anti-TRPV4 (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of TRPV4 in rat DRG. Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) antibody. A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.

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  Expression of TRPV4 in human U-87 cells. Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using Anti-TRPV4 (extracellular) antibody, (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).

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  Expression of TRPV4 in human U-87 cells. Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) antibody, (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4. 3rd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel, subfamily V, member 4
• Database Link:
  NP_076460
  Entrez Gene 59341 HumanEntrez Gene 66026 Rat
  UniProt ID Q9ERZ8
• Background: TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP). The TRPV subfamily consists of six members, TRPV1-6. TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.
• Synonyms: OTRPC4; TRP12; VR-OAC; VRL-2; VRL2; VROAC
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.