Ano1 Rabbit Polyclonal Antibody

CAT#: TA328784

Rabbit Polyclonal Anti-Anoctamin-1 (extracellular)

Product Images

Western blot analysis of rat dorsal root ganglion (DRG) lysate: 1. Anti-Anoctamin-1 (extracellular) antibody, (1:200). 2. Anti-Anoctamin-1 (extracellular) antibody, preincubated with the control peptide antigen.

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  Western blot analysis of rat small intestine lysate: 1. Anti-Anoctamin-1 (extracellular) antibody, (1:200). 2. Anti-Anoctamin-1 (extracellular) antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1. 3rd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anoctamin 1, calcium activated chloride channel
• Database Link:
  NP_848757
  Entrez Gene 309135 Rat
  UniProt ID Q8BHY3
• Background: Anoctamin (ANO, or TMEM16) is a family of membrane proteins which includes 10 members. This family is named so because these channels selective to ANions and have eight (OCT) transmembrane domains. Also, these channels are subject to glycosylation in their extracellular loops and have both intracellular N- and C-termini. Members of this family are expressed in a broad range of different organisms ranging from mammals, flies, worms, plants as well as yeast. Alternative splicing is known to affect these channels and regarding their oligomerization state, homedimerization has been observed although when heterologously expressed, these channels may hetero oligomerize. Ano1 (or TMEM16A, DOG1 and others) the first member to be identified was found to be a Ca2+-activated Cl channel therefore other members are likely to also be Cl channels. These channels are expressed in many different tissues: bronchiolar epithelial cells, pancreatic acinar cells, proximal kidney tubule epithelium, retina, dorsal root ganglia and submandibular gland. In fact, Ano1 gained a lot of attention as its activation may serve as a therapeutic treatment for cystic fibrosis since it is also expressed in the airways. These Ca2+-activated Cl channels are believed to play a role in development as knock out of Ano1 in mice causes abnormal development of the trachea. Ano2 (TMEM16B) has been shown to mediate Ca2+-activated Cl current in olfactory epithelium and photoreceptor synapses. Although relatively newly discovered channels, they are being discovered in many medical indications. Ano1 has become a marker in gastrointestinal tumors as its expression is significantly upregulated in such tumors. Similarly, Ano1 is also highly expressed other carcinomas.
• Synonyms: DOG1; FLJ10261; ORAOV2; TAOS2; TMEM16A