Clic5 Rabbit Polyclonal Antibody

CAT#: TA328786

Rabbit Polyclonal Anti-CLIC5

Product Images

Western blot analysis of mouse brain lysate (lanes 1 and 3), and rat lung lysate (lanes 2 and 4): 1-2. Anti-CLIC5 antibody, (1:200). 3-4. Anti-CLIC5 antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161- 174 of rat CLIC5. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: chloride intracellular channel 5
• Database Link:
  NP_446055
  Entrez Gene 224796 MouseEntrez Gene 94272 Rat
  UniProt ID Q9EPT8
• Background: Chloride (Cl) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity; they may reside in either the plasma membrane or the membrane of intracellular compartments or both. They are detected in the kidney, placenta, intestines, brain and the inner-ear. The vertebrate Cl intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6); each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl. CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene, has first been isolated from human placenta, and was shown, together with CLIC4 (with which it shares 76% homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton. CLIC5 shares about 40% homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear. Mice mutated in the Clic5 gene lack coordination and gradually become deaf, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor.
• Synonyms: CLIC5B; dJ447E21.4; FLJ90663; MST130; MSTP130