Atp2b2 Rabbit Polyclonal Antibody

CAT#: TA328787

Rabbit Polyclonal Anti-PMCA2

Product Images

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1, 2. Anti-PMCA2 antibody, (1:400). 3, 4. Anti-PMCA2 antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2. 2nd intracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATPase plasma membrane Ca2+ transporting 2
• Database Link:
  NP_036640
  Entrez Gene 11941 MouseEntrez Gene 24215 Rat
  UniProt ID P11506
• Background: The plasma membrane Ca2+ ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells. The PMCA and the Na+ calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations. Since it transports Ca2+ into the extracellular space, the PMCA is also an important regulator of the Ca2+ concentration in the extracellular space. The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain. The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, so it is suited for maintaining Ca2+ at its normally very low levels. The NCX is better suited for removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other.
• Synonyms: OTTHUMP00000158863; OTTHUMP00000178537; PMCA2; PMCA2a; PMCA2i