Ednrb Rabbit Polyclonal Antibody

CAT#: TA328804

Rabbit Polyclonal Anti-ET-B

Product Images

Western blot analysis of rat brain membranes: 1. Anti-Endothelin Receptor B antibody, (1:200). 2. Anti-Endothelin Receptor B antibody, preincubated with the control peptide antigen.

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  Expression of ET-B in rat lung. Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor B antibody (1:100). ET-B is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Note that vascular smooth muscle is not stained (blue arrows). Hematoxilin is used as the counterstain.

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  Expression of ET-B in rat thalamus. Immunohistochemical staining of rat dorsal peri-ventricular thalamus using Anti-Endothelin Receptor B antibody. Cresyl violet is used as the counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B. 3rd intracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: endothelin receptor type B
• Database Link:
  NP_059029
  Entrez Gene 1910 HumanEntrez Gene 13618 MouseEntrez Gene 50672 Rat
  UniProt ID P21451
• Background: Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein coupled receptors, ETAR and ETBR. Both ETAR and ETBR are present in the heart and in human myocardium at similar levels. The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity. Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities. Currently there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases. Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ETAR and ETBR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane. Hypermethylation of the ETBR correlated with transcriptional down-regulation and reduced expression of ETB receptor was observed in several prostate, bladder and colon cancer cell lines.
• Synonyms: ABCDS; ET-B; ETB; ETBR; ETRB; HSCR; HSCR2