Gabra1 Rabbit Polyclonal Antibody

CAT#: TA328809

Rabbit Polyclonal Anti-GABA (A) alpha1 Receptor (extracellular)

Product Images

Western blot analysis of rat brain lysates: 1. Anti-GABA(A) a1 Receptor (extracellular) antibody, (1:1000). 2. Anti-GABA(A) a1 Receptor (extracellular) antibody preincubated with the control peptide antigen.

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  Expression of GABA(A) a1 Receptor in mouse hippocampus. Immunohistochemical staining of GABA(A) a1 Receptor in mouse hippocampus using Anti-GABA(A) a1 Receptor (extracellular) antibody. A. Distribution of GABA(A) a1 Receptor (red) in mouse hippocampus. B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABA(A) α1 Receptor is restricted to neurons and their processes. DAPI is used as the counterstain (blue).

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  IHC staining of rat cerebellum using Anti-CaV1.2 antibody and Anti-GABA(A) a1 Receptor (extracellular) antibody. A. CaV1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABA(A) a1 Receptor (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between CaV1.2 and GABA(A) a1 Receptor in the granule layer.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABA (A) a1 Receptor. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor alpha1 subunit
• Database Link:
  NP_899155
  Entrez Gene 14394 MouseEntrez Gene 29705 Rat
  UniProt ID P62813
• Background: GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consists of 2a, 2Ã?, and 1d subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes. The a1 subunit is highly expressed in adulthood while the a2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the a-subunits that are highly expressed in early development (a2, a3, and a5) and those expressed in adulthood (a1) is suggested to play a major role in the development of temporal lobe epilepsy.
• Synonyms: ECA4; EJM; EJM5
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.