Gabra4 Rabbit Polyclonal Antibody

CAT#: TA328815

Rabbit Polyclonal Anti-GABA(A) alpha4 (extracellular)

Product Images

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and CCF-STGI cell line lysate (lanes 3 and 6): 1-3. Anti-GABA(A) a4 Receptor (extracellular) antibody, (1:200). 4-6. Anti-GABA(A) a4 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  IHC staining of rat cerebellum using Anti-GABA(A) a4 Receptor (extracellular) antibody. A GABARA4 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates expression of GABARA4 around the soma of Purkinje cells. DAPI is used as the counterstain (blue).

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  Expression of GABA(A) a4 Receptor in rat PC12 cells. Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-GABA(A) a4 Receptor (extracellular) antibody, (1:50), (red). B. Merge of A with the live view of the cell.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABA(A) a4 with replacement of C44 with serine. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor alpha4 subunit
• Database Link:
  NP_542154
  Entrez Gene 2557 HumanEntrez Gene 14397 MouseEntrez Gene 140675 Rat
  UniProt ID P28471
• Background: GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consist of 2a, 2Ã?, and 1? subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes. For example, the a4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain.
• Synonyms: GABRA4
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.