Gabrg1 Rabbit Polyclonal Antibody

CAT#: TA328817

Rabbit Polyclonal Anti-GABA(A) gamma1 Receptor

Product Images

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1. Anti-GABA(A) ?1 Receptor antibody, (1:500). 2. Anti-GABA(A) ?1 Receptor antibody, preincubated with the control peptide antigen.

• 

  Expression of GABA(A) ?1 Receptor in rat DRG. Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-GABA(A) ?1 Receptor antibody, followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Staining (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counterstain. C. merged image of A and B.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A)?1 Receptor. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor gamma 1 subunit
• Database Link:
  NP_542153
  Entrez Gene 14405 MouseEntrez Gene 140674 Rat
  UniProt ID P23574
• Background: The neurotransmitter GABA (?-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily.The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.They are composed of heteropentamer, with all of the five subunits contributing to the pore formation. To date, eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?. The native GABAA receptor, in most cases, consists of 2a, 2Ã? and 1? subunit. Three ? subunits genes have been identified in mammals.mRNA of the ?1 subunit is detected in the amygdala and septum.
• Synonyms: DKFZp686H2042; MGC33838