Mc3r Rabbit Polyclonal Antibody

CAT#: TA328872

Rabbit Polyclonal Anti-Melanocortin Receptor 3 (extracellular)

Product Images

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1, 2. Anti-Melanocortin Receptor 3 (extracellular) antibody, (1:200). 3, 4. Anti-Melanocortin Receptor 3 (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of MC3R in rat brain. Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Melanocortin Receptor 3 (extracellular) antibody, (1:100). Expression (green fluorescence) appears in the hypothalamic periventricular region (horizontal arrow), in some nerve cells (vertical arrow), and in the neuropil (asterisk). Hoechst 33342 is used as the counterstain (blue).

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  Indirect flow cytometry analysis in human promyelocytic leukemia HL-60 cells. black line, Cells + FITC-conjugated goat anti-rabbit antibody. green ling, Cells + Anti-Melanocortin Receptor 3 (extracellular) antibody, (1:40) + FITC conjugated goat anti-rabbit antibody.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)NSDSLTLEDQFIQHMD, corresponding to amino acid residues 102-117 of rat MC3R. 1st extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: melanocortin 3 receptor
• Database Link:
  NP_001020441
  Entrez Gene 4159 HumanEntrez Gene 17201 MouseEntrez Gene 29310 Rat
  UniProt ID P32244
• Background: Melanocortin Receptor 3 (MC3R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the a-, Ã?-, and ?-melanocyte-stimulating hormone (a-, Ã?-, ?-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC). One of the salient features of the melanocortin signaling system is the presence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP). All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with differing affinities. MC3R is the only one of the melanocortin receptors that shows no difference in binding specificity for any of the melanocortins, binding all of them with equal efficiency. AGRP is the high affinity endogenous antagonist of MC3R. The physiological function of MC3R is still poorly understood. The distribution of MC3R in hypothalamic nuclei of the central nervous system (CNS) suggests a role in the regulation of energy homeostasis. Indeed, studies with MC3R knockout (MC3R KO) mice showed that these mice have increased body fat due to increased feed efficiency. In humans, several polymorphisms of the MC3R gene are associated with high insulin levels and obesity in children. In addition, expression of MC3R in macrophages is thought to mediate the well known anti-inflammatory effects of a-MSH.
• Synonyms: BMIQ9; MC3; MC3-R; OB20; OQTL