Syp Rabbit Polyclonal Antibody

CAT#: TA328896

Rabbit Polyclonal Anti-Synaptophysin

Product Images

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat PC12 pheochromocytoma cell line lysate (lanes 3 and 6): 1-3. Anti-Synaptophysin antibody, (1:400). 4-6. Anti-Synaptophysin antibody, preincubated with the control peptide antigen.

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  Expression of Synaptophysin in rat DRG. Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Synaptophysin antibody, (1:100). Synaptophysin (red) is expressed in DRG neurons. Hoechst 33342 (blue) shows nuclear staining and is used as the counterstain.

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  Colocalization of NaV1.8 and Synaptophysin in rat DRG. Immunohistochemical staining of rat DRG frozen section using Anti-NaV1.8-ATTO-594 antibody and Anti-Synaptophysin antibody. A. NaV1.8 staining (red). B. Synaptophysin staining (green). C. Merged image demonstrates a partial overlap in the distribution of Nav1.8 and Synaptophysin within the DRGs. DAPI is used as the counterstain (blue).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin. 2nd intravesicular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: synaptophysin
• Database Link:
  NP_036796
  Entrez Gene 20977 MouseEntrez Gene 24804 Rat
  UniProt ID P07825
• Background: Synaptophysin is the major integral membrane protein of small synaptic vesicles. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined. Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons. Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles. Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal.
• Synonyms: MRX; MRXSYP; synaptophysin