Ntrk1 Rabbit Polyclonal Antibody

CAT#: TA328905

Rabbit polyclonal Anti-TrkA (extracellular)

Product Images

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-TrkA (extracellular) antibody, (1:200). 3, 4. Anti-TrkA (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of TrkA in rat DRG. Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular) antibody, (1:100). TrkA (red staining) is expressed in DRG neurons and in satellite microglia (arrows). Hoechst 33342 is used as the counterstain.

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  Expression of TrkA in rat brain hippocampal dentate gyrus. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections. A. Brain sections were stained using Anti-TrkA (extracellular) antibody, (1:1000), (green staining). B. The same section was also stained for glial fibrillary acidic protein (GFAP) (red and counterstained blue). C. Overlay of A and B demonstrates co-localization of TrkA and GFAP in dentate gyrus astrocytes.

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  Expression of TrkA in live intact rat PC12 cells. Immunocytochemical staining of live intact rat PC12 cells. A. Cells were stained with Anti-TrkA (extracellular) antibody (1:50), followed by goat anti-rabbit-AlexaFluor-494 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

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  Indirect flow cytometry analysis of live intact Jurkat (human T cell leukemia) cell line: black line: Cells + goat-anti-rabbit-Cy5. Green line: Cells + Anti-TrkA (extracellular) antibody, (1:25) + goat-anti-rabbit-Cy5.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA . Extracellular, 2nd immunoglobulin-like domain.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neurotrophic receptor tyrosine kinase 1
• Database Link:
  NP_067600
  Entrez Gene 4914 HumanEntrez Gene 18211 MouseEntrez Gene 59109 Rat
  UniProt ID P35739
• Background: NGF was the first neurotrophin to be discovered, more than 50 years ago. Three other proteins have been added to the list: BDNF, NT-3 and NT-4 (NT-4/5). These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kinase (TrK) receptors are receptor tyrosine kinases (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC1.
• Synonyms: DKFZp781I14186; MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.