Slc9a2 Rabbit Polyclonal Antibody

CAT#: TA328913

Rabbit polyclonal Anti-Na+/H+ Exchanger 2 (NHE-2)

Product Images

Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysate (lanes 2 and 5) and rat stomach lysate (lanes 3 and 6): 1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, (1:200). 4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, preincubated with the control peptide antigen.

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  Western blot analysis of human Colo-205 colorectal carcinoma cell lysates: Western blot analysis of human Colo-205 colorectal carcinoma cell lysates: 1. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, (1:200). 2. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, preincubated with the control peptide antigen.

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  Expression of Na+/H+ Exchanger 2 (NHE-2) in rat colon. Immunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2 . Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: solute carrier family 9 member A2
• Database Link:
  NP_036785
  Entrez Gene 6549 HumanEntrez Gene 226999 MouseEntrez Gene 24783 Rat
  UniProt ID P48763
• Background: In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+Â exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+,K+ ions inhibit NHE-1 but have no effect on NHE-2.The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated.NHE-2 is detected in the intestine, kidney and parietal cells.It is also detected in skeletal muscle and testis.
• Synonyms: NHE-2; NHE2