Kcnab2 Rabbit Polyclonal Antibody

CAT#: TA328972

Rabbit polyclonal Anti-KVbeta2

Product Images

Western blot analysis of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4): 1, 2. Anti-Kvβ2 antibody, (1:200). 3, 4. Anti-Kvβ2 antibody, preincubated with the control peptide antigen.

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  Western blot analysis of human Jurkat T cells: 1. Anti-Kvβ2 antibody, (1:200). 2. Anti-Kvβ2 antibody, preincubated with the control peptide antigen.

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  Expression of Kvβ2 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum using Anti-Kvβ2 antibody. A. Kvβ2 appears adjacent to Purkinje cells (green). B. Staining of GABAergic cells with mouse anti parvalbumin (PV, red). C. Confocal merge of Kvβ2 and PV demonstrates presence of Kvβ2 adjacent to Purkinje cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat KvÃ?2 . N-terminal part.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: potassium voltage-gated channel subfamily A regulatory beta subunit 2
• Database Link:
  NP_059000
  Entrez Gene 8514 HumanEntrez Gene 16498 MouseEntrez Gene 29738 Rat
  UniProt ID P62483
• Background: KvÃ?2 (or KCNAB2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are KvÃ?1 and KvÃ?3.The KvÃ? subunits were originally identified using a biochemical approach that demonstrated that the ion-conducting a subunits existed in a macromolecular complex with auxiliary Ã? subunits with a probable stoichiometry of a4Ã?4. It is now widely established that the regulatory Ã? subunits are able to alter both the biophysical (i.e. acceleration of inactivation kinetics) and biochemical (promote cell surface expression) properties of the functional Kv channel.The KvÃ? regulatory subunits are cytosolic proteins with conserved C-termini and variable N-terminus domains. The interaction with the a subunits is via the conserved C-terminal end that binds to a specific sequence in the N-terminus domain of the a subunits.KvÃ?2, as well as the other KvÃ? subunits can bind to all the members of the large Kv1.x (Shaker) channel subfamily. However, recent evidence suggests that it can also bind to members of the Kv4.x subfamily.KvÃ?2 is the most abundant Ã? subunit in the brain where it couples with all the Kv1.x subunits, but the protein is also expressed in peripheral tissues including heart, lung and cells of hematopoietic origin such as T cells where it couples with Kv1.3, the dominant voltage-dependent K+ channel in these cells. We have now developed a highly specific antibody directed against a unique region in the N-terminus end of the rat KvÃ?2 protein. The antibody will also recognize KvÃ?2 samples from mouse and human origin.
• Synonyms: AKR6A5; HKvbeta2; HKvbeta2.1; HKvbeta2.2; KCNA2B; KCNK2; KV-BETA-2; MGC117289; OTTHUMP00000000874; OTTHUMP00000000875; OTTHUMP00000000877