Kcnma1 Rabbit Polyclonal Antibody

CAT#: TA328989

Rabbit polyclonal Anti-KCa1.1 (BKCa) (extracellular)

Product Images

Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): 1, 2. Anti-KCa1.1 (BKCa) (extracellular) antibody, (1:200). 3, 4. Anti-KCa1.1 (BKCa) (extracellular) antibody, preincubated with the control peptide antigen.

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  Indirect flow cytometry analysis of live intact THP-1 (human acute monocytic leukemia cells) cell line: black line: Cells + goat-anti-rabbit-Cy5. green line: Cells + Anti-KCa1.1 (BKCa) (extracellular) antibody, (1:20) + goat-anti-rabbit-Cy5.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DSSNPIES(S)QNFYKD, corresponding to amino acid residues 199-213 of rat KCa1.1 . 1st extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: potassium calcium-activated channel subfamily M alpha 1
• Database Link:
  NP_114016
  Entrez Gene 3778 HumanEntrez Gene 16531 MouseEntrez Gene 83731 Rat
  UniProt ID Q62976
• Background: The KCa1.1 channel (also known as BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain. KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect between intracellular Ca2+ signaling pathways and membrane excitability. Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.
• Synonyms: bA205K10.1; BKTM; DKFZp686K1437; hSlo; K(VCA)alpha; KCa1.1; KCNMA; MaxiK; MGC71881; mSLO1; SAKCA; SLO; SLO-ALPHA; Slo1