Aqp3 Rabbit Polyclonal Antibody

CAT#: TA329015

Rabbit polyclonal Anti-Aquaporin 3

Product Images

Western blot analysis of rat kidney membranes: 1. Anti-Aquaporin 3 antibody, (1:200). 2. Anti-Aquaporin 3 antibody, preincubated with the control peptide antigen.

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  Expression of Aquaporin 3 in rat colon. Immunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 antibody, (brown). Aquaporin 3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. mucosa (M), submucosa (SM) and muscularis externa (ME).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: aquaporin 3
• Database Link:
  NP_113891
  Entrez Gene 360 HumanEntrez Gene 11828 MouseEntrez Gene 65133 Rat
  UniProt ID P47862
• Background: Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10. Little is known about the function of the two newest members, AQP-11 and AQP-12. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores. AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis. Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.
• Synonyms: AQP-3; GIL