Scn1a Rabbit Polyclonal Antibody

CAT#: TA329027

Rabbit polyclonal Anti-NaV1.1

Product Images

Western blot analysis of rat brain membranes: 1. Anti-Nav1.1 antibody, (1:200). 2. Anti-Nav1.1 antibody, preincubated with the control peptide antigen.

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  Expression of Nav1.1 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum using Anti-Nav1.1 antibody. A. The distribution of Nav1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of Nav1.1 and Parvalbumin.

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  Expression of Nav1.1 in rat DRG cells Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) using Anti-Nav1.1 antibody, (1:200), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat Nav1.1. Â ?Intracellular loop between domains I and II.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sodium voltage-gated channel alpha subunit 1
• Database Link:
  NP_110502
  Entrez Gene 6323 HumanEntrez Gene 20265 MouseEntrez Gene 81574 Rat
  UniProt ID P04774
• Background: Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability. Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9. The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9). Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit. Nav1.1 is a highly tetrodotoxin-sensitive channel and is broadly expressed in neurons. Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI).
• Synonyms: FEB3; FEB3A; GEFSP2; HBSCI; NAC1; Nav1.1; SCN1; SMEI