Scn3a Rabbit Polyclonal Antibody

CAT#: TA329030

Rabbit polyclonal Anti-NaV1.3

Product Images

Western blot analysis of rat newborn brain membranes: 1. Anti-Nav1.3 antibody, (1:200). 2. Anti-Nav1.3 antibody, preincubated with the control peptide antigen.

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  Expression of Nav1.3 in rat embryo DRG. Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-Nav1.3 antibody, (1:100). Nav1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 µm.

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  Expression of Nav1.3 in rat DRG primary cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglia (DRG) primary culture. A. DRG cells were stained using Anti-Nav1.3 antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat Nav1.3.? ? Intracellular, loop between domains I and II.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sodium voltage-gated channel alpha subunit 3
• Database Link:
  NP_037251
  Entrez Gene 6328 HumanEntrez Gene 20269 MouseEntrez Gene 497770 Rat
  UniProt ID P08104
• Background: Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability. Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav α subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9). Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit. Nav1.3 is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents. Up-regulation of Nav1.3 channel expression was described in injured neurons and injured spinal cord.
• Synonyms: KIAA1356; NAC3; Nav1.3