Scn10a Rabbit Polyclonal Antibody

CAT#: TA329039

Rabbit polyclonal Anti-NaV1.8

Product Images

Western blot analysis of rat DRG lysates: 1. Anti-Nav1.8 antibody, (1:200). 2. Anti-Nav1.8 antibody, preincubated with the control peptide antigen.

• 

  Expression of Nav1.8 in rat DRG. Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Nav1.8 antibody. Nav1.8 staining (red) is cytoplasmic and the intensity varies among DRG cells. There is a partial overlap in the distribution of Nav1.8 and neurofilament 200 (green).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat Nav1.8. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sodium voltage-gated channel alpha subunit 10
• Database Link:
  NP_058943
  Entrez Gene 6336 HumanEntrez Gene 29571 Rat
  UniProt ID Q62968
• Background: Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels. Expression of the α subunit isoform is developmentally and tissue specific. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SNS, SCN10A and PN3) is mainly expressed in small-diameter DRG neurons. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both, mRNA and protein, in Purkinje cells of MS patients and also in animal models.
• Synonyms: hPN3; Nav1.8; PN3; SNS