UBE2N Rabbit Polyclonal Antibody

CAT#: TA332104

Rabbit Polyclonal Anti-UBE2N Antibody

Product Images

Sample Type: 1. Human NT-2 cells (60ug); 2. mouse brain extracts (80ug); Primary Antibody Dilution: 2ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20, 000; Image Submitted by: Yuzhi Chen; U

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  Lanes: 1: 40ng HIS-UBE2D1 protein; 2: 40ng HIS-UBE2D2 protein; 3: 40ng HIS-UBE2D3 protein; 4: 40ng HIS-UBE2D4 protein; 5: 40ng HIS-UBE2E1 protein; 6: 40ng HIS-UBE2E2 protein; 7: 40ng HIS-UBE2E3 protein; 8: 40ng HIS-UBE2K protein; 9: 40ng HIS-UBE2L3 protei

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  WB Suggested Anti-UBE2N Antibody Titration: 2.5ug/ml; Positive Control: Jurkat cell lysateUBE2N is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

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  Sample Type. Mouse brain stem cells Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody: ilution. 1: 1000; Color/Signal Descriptions. UBE2N: Red DAPI: Blue; Gene Name: UBE2N; Submitted by: Dr. Yuz

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB, IHC
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for Anti-UBE2N Antibody: synthetic peptide directed towards the middle region of human UBE2N. Synthetic peptide located within the following region: GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNE
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17 kDa
• Gene Name: ubiquitin conjugating enzyme E2 N
• Database Link:
  NP_003339
  Entrez Gene 93765 MouseEntrez Gene 7334 Human
  UniProt ID P61088
• Background: UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
• Synonyms: HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN
• Note: Immunogen sequence homology: Dog: 100%; Pig: 100%; Rat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Sheep: 100%; Yeast: 100%; Bovine: 100%; Rabbit: 100%; Zebrafish: 100%; Guinea pig: 100%; Goat: 93%

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis