Rb (RB1) Rabbit Polyclonal Antibody

CAT#: TA332369

Rabbit anti-RB1 Polyclonal Antibody

Product Images

Western blot analysis of Jurkat cell and A431 cell using RB1 antibody.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human RB1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110 kDa
• Gene Name: RB transcriptional corepressor 1
• Database Link:
  NP_000312
  Entrez Gene 5925 Human
  UniProt ID P06400
• Background: The retinoblastoma tumor suppressor protein, RB1, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). RB1 has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits RB1 target binding and allows cell cycle progression (5). RB1 inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
• Synonyms: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer