PARP1 Rabbit Polyclonal Antibody

CAT#: TA332370

Rabbit anti-PARP1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of Jurkat cell line, using PARP1 antibody.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:1000 - 1:2000;IHC 1:20 – 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human PARP1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 1014
• Gene Name: poly(ADP-ribose) polymerase 1
• Database Link:
  NP_001609
  Entrez Gene 11545 MouseEntrez Gene 25591 RatEntrez Gene 142 Human
  UniProt ID P09874
• Background: PARP1, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP1, the cleavage occurs between Asp214 and Gly215, which separates the PARP1 amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP1 helps cells to maintain their viability; cleavage of PARP1 facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
• Synonyms: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair