HSP27 (HSPB1) Rabbit Polyclonal Antibody

CAT#: TA332377

Rabbit anti-HSPB1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of HUVEC cells, using HSPB1 antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human HSPB1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 205
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  NP_001531
  Entrez Gene 15507 MouseEntrez Gene 24471 RatEntrez Gene 3315 Human
  UniProt ID P04792
• Background: Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSPB1 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSPB1 increases several-fold to confer cellular resistance to the adverse environmental change. HSPB1 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSPB1 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSPB1 modulates actin polymerization and reorganization (5,6).
• Synonyms: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway