PCNA Rabbit Polyclonal Antibody

CAT#: TA332379

Rabbit polyclonal anti-PCNA antibody, Loading control

Product Images

Western blot analysis of extracts of various cell lines, using PCNA antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse, Rat, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PCNA
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 261
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  NP_002583
  Entrez Gene 18538 MouseEntrez Gene 25737 RatEntrez Gene 718006 MonkeyEntrez Gene 5111 Human
  UniProt ID P12004
• Background: Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
• Synonyms: ATLD2

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair