SOX2 Rabbit Polyclonal Antibody

CAT#: TA332386

Rabbit anti-SOX2 Polyclonal Antibody

Product Images

Western blot analysis of brain cell lysate using SOX2 antibody.

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  Immunohistochemistry of paraffin-embedded rat brain using SOX2 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded mouse brain using SOX2 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat brain using SOX2 antibody at dilution of 1:100 (x400 lens).

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  Immunofluorescence analysis of MCF7 cell using SOX2 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SOX2
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: SRY-box 2
• Database Link:
  NP_003097
  Entrez Gene 20674 MouseEntrez Gene 499593 RatEntrez Gene 6657 Human
  UniProt ID P48431
• Background: Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
• Synonyms: ANOP3; MCOPS3

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors