beta Arrestin 1 (ARRB1) Rabbit Polyclonal Antibody

CAT#: TA332389

Rabbit anti-ARRB1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ARRB1 antibody.

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  Immunohistochemistry of paraffin-embedded human liver cancer using ARRB1 antibody at dilution of 1:100 (200x lens).

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  Immunohistochemistry of paraffin-embedded mouse testis using ARRB1 antibody at dilution of 1:100 (200x lens).

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  Immunohistochemistry of paraffin-embedded rat lung using ARRB1 antibody at dilution of 1:100 (200x lens).

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  Immunofluorescence analysis of HeLa cell using ARRB1 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:20- 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ARRB1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 418
• Gene Name: arrestin beta 1
• Database Link:
  NP_064647
  Entrez Gene 25387 RatEntrez Gene 109689 MouseEntrez Gene 408 Human
  UniProt ID P49407
• Background: Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
• Synonyms: ARB1; ARR1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Endocytosis, MAPK signaling pathway