DNA Polymerase iota (POLI) Rabbit Polyclonal Antibody

CAT#: TA332469

Rabbit anti-POLI Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using POLI antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human POLI
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80 kDa
• Gene Name: polymerase (DNA) iota
• Database Link:
  NP_009126
  Entrez Gene 26447 MouseEntrez Gene 11201 Human
  UniProt ID Q9UNA4
• Background: Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates â??Gâ? opposite template â??Tâ? more frequently than incorporates the correct â??A.â? _ It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. _. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress. _
• Synonyms: RAD3OB; RAD30B

Reference Data

• Protein Families: Druggable Genome