SMAD5 Rabbit Polyclonal Antibody

CAT#: TA332472

Rabbit anti-SMAD5 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SMAD5 antibody.

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  Immunohistochemistry of paraffin-embedded mouse brain using Smad5 antibody.

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  Immunohistochemistry of paraffin-embedded rat brain using Smad5 antibody.

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  Immunohistochemistry of paraffin-embedded rat kidney using Smad5 antibody.

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  Immunohistochemistry of paraffin-embedded human esophagus using Smad5 antibody.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:100 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human SMAD5
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52 kDa
• Gene Name: SMAD family member 5
• Database Link:
  NP_005894
  Entrez Gene 17129 MouseEntrez Gene 59328 RatEntrez Gene 4090 Human
  UniProt ID Q99717
• Background: Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).
• Synonyms: DWFC; JV5-1; MADH5

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway