AIF (AIFM1) Rabbit Polyclonal Antibody

CAT#: TA332560

Rabbit anti-AIFM1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using AIFM1 antibody.

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  Immunohistochemistry of paraffin-embedded human esophageal using AIFM1 antibody at dilution of 1:100 (x400 lens).

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  Immunohistochemistry of paraffin-embedded rat lung using AIFM1 antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of U2OS cell using AIFM1 antibody. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cell using AIFM1 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200;IP 1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human AIFM1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66 kDa
• Gene Name: apoptosis inducing factor, mitochondria associated 1
• Database Link:
  NP_665811
  Entrez Gene 26926 MouseEntrez Gene 83533 RatEntrez Gene 9131 Human
  UniProt ID O95831
• Background: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
• Synonyms: AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis