DDX5 Rabbit Polyclonal Antibody

CAT#: TA332589

Rabbit anti-DDX5 Polyclonal Antibody

Product Images

Western blot analysis of extracts of Mouse spleen, using DDX5 antibody (TA332589) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB,1:500 - 1:1000
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69kDa
• Gene Name: DEAD-box helicase 5
• Database Link:
  NP_004387
  Entrez Gene 13207 MouseEntrez Gene 1655 Human
  UniProt ID P17844
• Background: This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants.
• Synonyms: G17P1; HLR1; HUMP68; p68

Reference Data

• Protein Pathways: Spliceosome