Ku80 (XRCC5) Rabbit Polyclonal Antibody

CAT#: TA332970

Rabbit anti-XRCC5 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using XRCC5 antibody.

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  Immunofluorescence analysis of A549 cell using XRCC5 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC-P,1:100 - 1:200
  IF/ICC,1:20 - 1:100
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83kDa
• Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 5
• Database Link:
  NP_066964
  Entrez Gene 22596 MouseEntrez Gene 363247 RatEntrez Gene 7520 Human
  UniProt ID P13010
• Background: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Synonyms: KARP-1; KARP1; KU80; Ku86; KUB2; NFIV

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Non-homologous end-joining