EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: TA333196

Phospho-EIF4EBP1-T36 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts from MDA-MB-435 cells, using Phospho-eIF4EBP1-T36 antibody (TA333196).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500-1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding T36 of human EIF4EBP1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  NP_004086
  Entrez Gene 13685 MouseEntrez Gene 116636 RatEntrez Gene 1978 Human
  UniProt ID Q13541
• Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
• Synonyms: 4E-BP1; 4EBP1; BP-1; PHAS-I

Reference Data

• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway