VEGF Receptor 2 (KDR) Rabbit Polyclonal Antibody

CAT#: TA336288

Rabbit Polyclonal VEGF R2/KDR/Flk-1 Antibody

Product Images

Western Blot: VEGF Receptor 2 Antibody TA336288 - Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using TA336288. ECL 10 second exposure.

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  Immunocytochemistry/Immunofluorescence: VEGF Receptor 2 Antibody TA336288 - VEGF Receptor 2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/ml, IF: 1:10
• Reactivities: Hamster, Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]
• Formulation: Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 150 kDa
• Gene Name: kinase insert domain receptor
• Database Link:
  NP_002244
  Entrez Gene 3791 Human
  UniProt ID P35968
• Background: VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulates angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands and binding of VEGFs to isoform 1 leads to multiple signaling cascades activation. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.
• Synonyms: CD309; FLK1; VEGFR; VEGFR2
• Note: This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, VEGF signaling pathway