FOXP3 Rabbit Polyclonal Antibody
Frequently bought together (2)
Transient overexpression lysate of forkhead box P3 (FOXP3), transcript variant 1
USD 605.00
Other products for "FOXP3"
Specifications
Product Data | |
Applications | FC, IHC, WB |
Recommended Dilution | Single Cell Western: 100 ug/mL, Western Blot: 1:2000, Flow Cytometry: 1:8000, Immunocytochemistry/ Immunofluorescence: 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin: 1:400, Immunoblotting, Immunohistochemistry: 1:400, Dual RNAscope ISH-IHC |
Reactivities | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6] |
Formulation | Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze. |
Concentration | lot specific |
Purification | Protein G purified |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Gene Name | forkhead box P3 |
Database Link | |
Background | FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. |
Synonyms | AIID; DIETER; IPEX; JM2; PIDX; XPID |
Note | This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. |
Reference Data | |
Protein Families | Transcription Factors |
Documents
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