FANCD2 Rabbit Polyclonal Antibody

CAT#: TA336310

Rabbit Polyclonal FANCD2 [p Ser222] Antibody

Product Images

Western Blot: FANCD2 [p Ser222] Antibody TA336310 - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)

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  Western Blot: FANCD2 [p Ser222] Antibody TA336310 - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 [p Ser222] Antibody (C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:100-1:500
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to human FANCD2 (residues 217-226), surrounding the phosphorylated Serine 222. [Swiss-Prot# Q9BXW9]
• Formulation: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Fanconi anemia complementation group D2
• Database Link:
  NP_001018125
  Entrez Gene 2177 Human
  UniProt ID Q9BXW9
• Background: FANCD2 (Fanconi anemia group D2 protein) belongs to family of FANC proteins which includes several members such as FANC-A, -B, -C, -D1/BRCA2, -D2, -E, -F, -G, -I, -J/BRIP1, -L, -M and -N/PALB2. FANCD2 interacts with several proteins such as FANCE/FANCI, USP1, MEN1, BRCA1, BRCA2, BLM, MTMR15/FAN1 and DCLRE1B/Apollo. FANC proteins (-A, -B, -C, -D1, -D2, -E, -F, -G, -L) cooperate in FANC/BRCA pathway resulting in monoubiquitination of FANCD2 which then promotes BRCA2/FANCD1 loading onto chromatin for creating nuclear foci at DNA damage sites. It is involved in DNA double-strand breaks repair, both by homologous recombination and single-strand annealing. FANCD2 is required for maintenance of chromosomal stability and promotes accurate/efficient pairing of homologs during meiosis. FANCD2 may participate in S phase/G2 phase checkpoint activation upon DNA damage amd plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 characterized by anemia, leukopenia and thrombopenia.
• Synonyms: FA-D2; FA4; FACD; FAD; FAD2; FANCD
• Note: This FANCD2 [p Ser222] antibody is useful for Western Blot. 1:400 dilution recommended for PD20 FANCD2 overexpressing cells, 1:100 for HeLa cells, 1:200 for other cell types.

Reference Data

• Protein Families: Druggable Genome