Ku80 (XRCC5) Rabbit Polyclonal Antibody

CAT#: TA336311

Rabbit Polyclonal Ku80/XRCC5 Antibody

Product Images

Immunoprecipitation: Ku80 Antibody TA336311 - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-503. A Ku80 deficient cell lysate, XRS, was used as a negative control.

Specifications

Product Data

• Applications: IP
• Recommended Dilution: WB: 1:100-1:2000
• Reactivities: Hamster, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to residues 323-338 [FSKVDEEQMKYKSEGK] of the 80 kDa Ku80 protein.
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 5
• Database Link:
  NP_066964
  Entrez Gene 22596 MouseEntrez Gene 363247 RatEntrez Gene 7520 Human
  UniProt ID P13010
• Background: Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends.
• Synonyms: KARP-1; KARP1; KU80; Ku86; KUB2; NFIV
• Note: This antibody has only been tested in Western blot. Recommended working dilution: WB at 1:500 dilution However, the investigator should determine the optimal dilution.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Non-homologous end-joining