Caspase-6 (CASP6) Rabbit Polyclonal Antibody

CAT#: TA336324

Rabbit Polyclonal Caspase-6 Antibody

Product Images

Western Blot: Caspase-6 Antibody TA336324 - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.

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  Immunohistochemistry: Caspase-6 Antibody TA336324 - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.

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  Immunohistochemistry-Paraffin: Caspase-6 Antibody TA336324 - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. Gemistocytoma (low grade tumor) at A) low and B) high magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:50-1:200, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin: 1:1000-1:5000, Immunohistochemistry-Frozen
• Reactivities: Human, Mouse, Rat, Canine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant catalytically active human Caspase-6 protein was used as immunogen.
• Formulation: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 6
• Database Link:
  NP_001217
  Entrez Gene 12368 MouseEntrez Gene 83584 RatEntrez Gene 839 Human
  UniProt ID P55212
• Background: Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis-inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
• Synonyms: MCH2
• Note: Immunohistochemistry (frozen): Users should optimize according to model and immunodetection system used (secondary reagents) Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reference Data

• Protein Families: Druggable Genome, Protease, Stem cell - Pluripotency
• Protein Pathways: Apoptosis