5HT3A receptor (HTR3A) Rabbit Polyclonal Antibody

CAT#: TA336339

Rabbit Polyclonal 5-HT3A Antibody

Product Images

Western Blot: 5-HT3A Antibody TA336339 - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.

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  The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim.

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  Simple Western: 5-HT3A Antibody TA336339 - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: 5-HT3A Antibody TA336339 - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 3 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry: 1:20-1:1000, Simple Western: 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE) of rat 5-HT3 Receptor, accession number P35563.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: 5-hydroxytryptamine receptor 3A
• Database Link:
  NP_998786
  Entrez Gene 15561 MouseEntrez Gene 79246 RatEntrez Gene 3359 Human
  UniProt ID P46098
• Background: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord.
• Synonyms: 5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane