CYLD Rabbit Polyclonal Antibody

CAT#: TA336362

Rabbit Polyclonal CYLD Antibody

Product Images

Immunocytochemistry/Immunofluorescence: CYLD Antibody TA336362 - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.

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  Simple Western: CYLD Antibody TA336362 - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: CYLD Antibody TA336362 - Staining of HeLa cell using this antibody at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.

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  Western Blot: CYLD Antibody TA336362 - Analysis of CYLD in HeLa cell lysate using this antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 5-10 ug/ml, Western Blot: 2-4 ug/ml, Simple Western: 1:50
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CYLD lysine 63 deubiquitinase
• Database Link:
  NP_001035814
  Entrez Gene 1540 Human
  UniProt ID Q9NQC7
• Background: CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
• Synonyms: BRSS; CDMT; CYLD1; CYLDI; EAC; MFT; MFT1; SBS; TEM; USPL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway