Osteoprotegerin (TNFRSF11B) Mouse Monoclonal Antibody [Clone ID: 98A1071]

CAT#: TA336370

Mouse Monoclonal Osteoprotegerin/TNFRSF11B Antibody (98A1071)

Product Images

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

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  Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

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  Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Staining of human heart tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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  Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Staining of human liver. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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  Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin antibody generated a diffused but weak cytoplasmic staining with a very specific and strong punctate signal at the periphery of the cells.

• Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) TA336370 - Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary.

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Specifications

Product Data

• Clone Name: 98A1071
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot: 1 ug/ml, Immunohistochemistry-Paraffin: 1:100-1:1000, Immunohistochemistry: 1:100-1:1000, CyTOF-ready, ELISA, Flow Cytometry: 0.2-1 ug/1 million cells, Immunocytochemistry/ Immunofluorescence: 1:20-1:1000, Immunohistochemistry-Frozen: 1:20-1:1000, Immunoprecipitation: 1:20-1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor receptor superfamily member 11b
• Database Link:
  NP_002537
  Entrez Gene 4982 Human
  UniProt ID O00300
• Background: Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily. As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease.
• Synonyms: OCIF; OPG; TR1
• Note: This antibody is useful in Flow Cytometry: 0.2-1 ug/10^6 cells Immunocytochemistry/Immunofluorescence: see Sandra 2006 Immunohistochemistry (frozen): see Boumans 2012 for details. Immunoprecipitation: see Sandra 2006

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction