IKK beta (IKBKB) Mouse Monoclonal Antibody [Clone ID: 10AG2]

CAT#: TA336374

Mouse Monoclonal IKK beta Antibody (10AG2)

Product Images

Simple Western: IKK beta Antibody (10AG2) TA336374 - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Flow Cytometry: IKK beta Antibody (10AG2) TA336374 - Analysis using the Alexa Fluor (R) 488 conjugate of TA336374. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control , red represents the IKKbeta antibody.

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  Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) TA336374 - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

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  Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) TA336374 - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Flow Cytometry: IKK beta Antibody (10AG2) TA336374 - Analysis of PE conjugate of TA336374. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) TA336374PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi

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  Flow Cytometry: IKK beta Antibody (10AG2) TA336374 - Analysis using Alexa Fluor (R) 647 conjugate of TA336374. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) TA336374 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Western Blot: IKK beta Antibody (10AG2) TA336374 - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.

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Specifications

Product Data

• Clone Name: 10AG2
• Applications: FC, IHC, WB
• Recommended Dilution: Immunoprecipitation: 1:10 - 1:500, Simple Western: 1:12.5, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, Immunohistochemistry: 1:10 - 1:500, Immunoblotting, CyTOF-ready, Flow Cytometry: 0.25 - 0.5 ug/ 10^6 cells, Western Blot: 2 - 4 ug/ml
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.564 kDa
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Database Link:
  NP_001547
  Entrez Gene 16150 MouseEntrez Gene 3551 Human
  UniProt ID O14920
• Background: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.
• Synonyms: IKK-beta; IKK2; IKKB; IMD15; NFKBIKB
• Note: Useful in Immunohistochemistry-Paraffin See Page et al.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus